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Image Search Results
Journal: Cell Death & Disease
Article Title: Aldolase B-driven lactagenesis and CEACAM6 activation promote cell renewal and chemoresistance in colorectal cancer through the Warburg effect
doi: 10.1038/s41419-023-06187-z
Figure Lengend Snippet: A Metabolomic analysis based on nuclear magnetic resonance (NMR) showing metabolite levels in CRC cells with or without ALDOB overexpression. Black dashed lines indicate relative metabolite levels in control-treated cells. B Lactate levels in medium from cells with control or ALDOB overexpression. C Transwell-based co-culture system for assessment of cell growth (left panel) and chemoresistance (right panels). D Supplementation of medium from cells with or without ALDOB overexpression for assessment of cell proliferation (left panel) and chemoresistance (right panels). E Western blots demonstrating levels of the indicated proteins in cells with or without ALDOB overexpression. The statistical analysis is displayed in the right panel. F Immunofluorescence assay showing the expression of exogenous ALDOB (ALDOB-MYC) and LDHB in CRC cells transfected with ALDOB expression plasmid. The scale bar represents 20 μm. G Western blots showing the levels of the indicated proteins in cells treated with different concentrations of lactate. H Immunofluorescence assay showing LDHB expression in CRC cells treated with indicated concentrations of lactate. The scale bar represents 20 μm. All P values were obtained using the paired two-tailed Student’s t -test. * P < 0.05; ** P < 0.01; *** P < 0.001.
Article Snippet: A mouse monoclonal antibody targeting the
Techniques: Nuclear Magnetic Resonance, Over Expression, Control, Co-Culture Assay, Western Blot, Immunofluorescence, Expressing, Transfection, Plasmid Preparation, Two Tailed Test